牡蛎粗多糖对脂多糖刺激仔猪NF-κB信号通路相关基因转录的影响

doi:10.11843/j.issn.0366-6964.2015.06.021

Abstract

Abstract:

This study was designed to explore the impact of Oyster Polysaccharides on immune stress piglets of NF-κB signaling pathway-related genes A total of thirty Duroc × Landrace × Yorkshire castrated piglets of 28±1 d were randomly allocated into five groups，namely Blank control (Ⅰ)，Immunological stress control (Ⅱ) and Oyster polysaccharide Low (Ⅲ)，Medium (Ⅳ) and High (Ⅴ) dose group，with six replicates per group according to the principle of similar weight.Piglets were fed basal diet (Control) or 0.5%，0.8%，1.2% OPS (OPS Low，Medium and High dose group) for 30 days.The piglets were injected i.p with a dose of Escherichia coli LPS (100 μg•kg^-1 BW) except the Blank control which were injected with the same dose of normal saline.Three hours later，the liver，spleen，adrenal gland，lymph nodes and thymus were collected for detecting the relative transcription level of TLR-4，p50 and p300.Results were as follows：(1) Compared with the GroupⅠ，p300 relative transcript levels of GroupⅡ were significantly decreased in Lymph Node，while the other indicators were significantly increased in various organs (P<0.01).The relative transcript levels of TLR-4 in lymph nodes，p50 in adrenal gland and p300 in liver，adrenal gland and lymph nodes were significantly decreased (P<0.01) while the relative transcript levels of p50 in adrenal gland and p300 in liver，adrenal gland and lymph node were significantly increased (P<0.01) in Group Ⅲ than GroupⅠ.The relative transcript levels of TLR-4 in lymph nodes and spleen，p300 in liver，adrenal gland and lymph nodes were significantly decreased (P<0.05 or P<0.01) while the relative transcript levels of TLR-4 in liver，adrenal gland and thymus and p50 in liver，thymus were significantly increased (P<0.05 or P<0.01) in Group Ⅳ than GroupⅠ.The relative transcript levels of TLR-4 in spleen，p300 in liver，lymph nodes，thymus and adrenal gland were significantly decreased (P<0.05 or P<0.01) while the relative transcript levels of TLR-4 in liver，adrenal gland and lymph nodes and p50 in liver，thymus and adrenal gland were significantly increased in Group Ⅴ than Group Ⅰ(P<0.05 or P<0.01).(2)Compared with the Group Ⅱ，the relative transcription of TLR-4 levels were significantly decreased in these organs of Group Ⅲ，Ⅳ and Ⅴ (P<0.05 or P<0.01)other than in lymph node of Group Ⅴ (P>0.05).The relative transcription of p50 levels were significantly decreased in adrenal glands，lymph glands and spleen of Group Ⅲ，Ⅳ，and Ⅴ(P<0.01)；and were significantly decreased in liver and significantly increased in thymus of Group Ⅲ(P<0.01)；and that were significantly increased in thymus of Group Ⅴ (P<0.01).The relative transcription of p300 mRNA levels were significantly decreased in liver，adrenal glands，thymus and spleen of Group Ⅲ，Ⅳ，and Ⅴ(P<0.01)；and that were significantly increased in lymph nodes of Group Ⅳ (P<0.01).Oyster polysaccharides can relieve stress piglets transcription of NF-κB signaling pathway-related genes.

CLC Number:

S852.4

HUANG Zhi-jian，LUO Gang，CHEN Teng-teng，JIANG He-ji，ZENG Xin-bin. Explore Mechanism of Oyster Crude Polysaccharide Alleviated Immune Stress on Weanling Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 1037-1046.

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Explore Mechanism of Oyster Crude Polysaccharide Alleviated Immune Stress on Weanling Piglets

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